HIV is different in structure from other retroviruses. It is roughly spherical
[54] with a diameter of about 120
nm, around 60 times smaller than a
red blood cell, yet large for a virus.
[55] It is composed of two copies of positive single-stranded
RNA that codes for the virus's nine
genes enclosed by a conical
capsid composed of 2,000 copies of the viral protein
p24.
[56] The single-stranded RNA is tightly bound to nucleocapsid proteins, p7 and enzymes needed for the development of the virion such as
reverse transcriptase,
proteases,
ribonuclease and
integrase. A matrix composed of the viral protein p17 surrounds the capsid ensuring the integrity of the virion particle.
[56] This is, in turn, surrounded by the
viral envelope that is composed of two layers of fatty molecules called
phospholipids taken from the membrane of a human cell when a newly formed virus particle buds from the cell. Embedded in the viral envelope are proteins from the host cell and about 70 copies of a complex HIV protein that protrudes through the surface of the virus particle.
[56] This protein, known as Env, consists of a cap made of three molecules called
glycoprotein (gp) 120, and a stem consisting of three
gp41 molecules that anchor the structure into the viral envelope.
[57] This glycoprotein complex enables the virus to attach to and fuse with target cells to initiate the infectious cycle.
[57] Both these surface proteins, especially gp120, have been considered as targets of future treatments or vaccines against HIV.
[58]
The RNA genome consists of at least seven structural landmarks (
LTR,
TAR, RRE, PE, SLIP, CRS, and INS) and nine genes (
gag,
pol, and
env,
tat,
rev,
nef,
vif,
vpr,
vpu, and
tev) encoding 19 proteins. Three of these genes,
gag,
pol, and
env, contain information needed to make the structural proteins for new virus particles.
[56] For example,
env codes for a protein called gp160 that is broken down by a viral enzyme to form gp120 and gp41. The six remaining genes,
tat,
rev,
nef,
vif,
vpr, and
vpu (or
vpx in the case of HIV-2), are regulatory genes for proteins that control the ability of HIV to infect cells, produce new copies of virus (replicate), or cause disease.
[56] The two Tat proteins (p16 and p14) are
transcriptional transactivators for the LTR promoter acting by binding the TAR RNA element. The TAR may also be processed into
microRNAs that regulate the
apoptosis genes
ERCC1 and
IER3.
[59][60] The
Rev protein (p19) is involved in shuttling RNAs from the nucleus and the cytoplasm by binding to the RRE RNA element. The Vif protein (p23) prevents the action of
APOBEC3G (a cell protein that deaminates DNA:RNA hybrids and/or interferes with the Pol protein). The Vpr protein (p14) arrests
cell division at G2/M. The Nef protein (p27) down-regulates
CD4 (the major viral receptor), as well as the
MHC class I and
class II molecules.
[61][62][63] Nef also interacts with SH3 domains. The Vpu protein (p16) influences the release of new virus particles from infected cells.
[56] The ends of each strand of HIV RNA contain an RNA sequence called the
long terminal repeat (LTR). Regions in the LTR act as switches to control production of new viruses and can be triggered by proteins from either HIV or the host cell. The
Psi element is involved in viral genome packaging and recognized by Gag and Rev proteins. The SLIP element (TTTTTT) is involved in the frameshift in the Gag-Pol reading frame required to make functional Pol
Postingan
